rabbit anti cd36 polyclonal igg  (Proteintech)

Journal:

Article Title: Macrophage-Specific Lipid-Based Nanoparticles Improve MRI Detection and Characterization of Human Atherosclerosis

doi: 10.1016/j.jcmg.2008.08.009

Figure Lengend Snippet: Confocal microscopy merged images (NBD=green, rhodamine=red, DAPI=blue) at 100X of activated human monocytes incubated in vitro with control (A), NBD-labeled untargeted NPs (B), rhodamine-labeled NPs targeting CD36 (C), and with both NBD-labeled untargeted NPs and rhodamine-labeled NPs targeting CD36 (D) and stained with DAPI. Included with each image in the bottom of the panel is the Gd concentration (μg/ml) normalized to the cell numbers present. As seen in panel A, the nuclei of the control cells appear blue and there was no significant GD present as expected. In cells incubated with untargeted NBD-labeled NPs (B), there was trivial green fluorescence seen and minimal uptake of Gd as seen on ICP-MS. Cells incubated with rhodamine-labeled NPs targeting C36 (C) demonstrated significant red fluorescence corresponding with cellular uptake of targeted NPs and ICP-MS also demonstrated significant Gd uptake. As seen in Panel D, there was again significant uptake of rhodamine-labeled NPs targeting CD36 but trivial uptake of untargeted NBD-labeled NPs. The cellular uptake of Gd as shown on ICP-MS in panel D appears similar to the sum of Gd concentrations for panels B and C.

Article Snippet: Biotinylated rabbit anti-human polyclonal antibodies to human CD36 (IgG, Novus Biologicals) and biotinylated ChromPure rabbit IgG, Fc fragment (Jackson Immunoresearch) were used to make targeted NPs s and Fc-NPs respectively.

Techniques: Confocal Microscopy, Incubation, In Vitro, Control, Labeling, Staining, Concentration Assay, Fluorescence

Journal:

Article Title: Macrophage-Specific Lipid-Based Nanoparticles Improve MRI Detection and Characterization of Human Atherosclerosis

doi: 10.1016/j.jcmg.2008.08.009

Figure Lengend Snippet: Pre- and Post-contrast SNR values and CNR values demonstrating the degree of aortic plaque enhancement with Gd-loaded NPs targeting CD36, Fc-NPs, and untargeted NPs. Numbers are expressed as Mean±SD

Article Snippet: Biotinylated rabbit anti-human polyclonal antibodies to human CD36 (IgG, Novus Biologicals) and biotinylated ChromPure rabbit IgG, Fc fragment (Jackson Immunoresearch) were used to make targeted NPs s and Fc-NPs respectively.

Techniques:

Journal:

Article Title: Macrophage-Specific Lipid-Based Nanoparticles Improve MRI Detection and Characterization of Human Atherosclerosis

doi: 10.1016/j.jcmg.2008.08.009

Figure Lengend Snippet: Comparison of pre- and post-contrast T1 images of aortas treated with Gd-loaded NPs targeting CD36 (A & B respectively), untargeted NPs (C & D respectively), and Fc-NPs (E & F respectively). Included in the bottom left corner is a scale bar that represents one centimeter in length. The error bar graph included below (Figure 2B) provides a graphical representation comparing preand post-contrast SNR seen between aorta sections incubated with targeted NPs, untargeted NPs, and Fc-NPs.

Article Snippet: Biotinylated rabbit anti-human polyclonal antibodies to human CD36 (IgG, Novus Biologicals) and biotinylated ChromPure rabbit IgG, Fc fragment (Jackson Immunoresearch) were used to make targeted NPs s and Fc-NPs respectively.

Techniques: Comparison, Incubation

Journal:

Article Title: Macrophage-Specific Lipid-Based Nanoparticles Improve MRI Detection and Characterization of Human Atherosclerosis

doi: 10.1016/j.jcmg.2008.08.009

Figure Lengend Snippet: Confocal microscopy images of aortic atherosclerotic plaque incubated with (A) NBD-labeled (green) NPs targeting CD36, (B) DAPI (blue), (C) RPE-labeled (red) anti-CD68 (macrophage lysosomes). The merged image (D) demonstrates co-localization of NDB-labeled NPs targeting CD36 (macrophage scavenger receptor) and RPE-labeled anti-CD68. The arrow points to an atheroma with high macrophage content and thin fibrous cap. (E) Corresponding light microscope image.

Article Snippet: Biotinylated rabbit anti-human polyclonal antibodies to human CD36 (IgG, Novus Biologicals) and biotinylated ChromPure rabbit IgG, Fc fragment (Jackson Immunoresearch) were used to make targeted NPs s and Fc-NPs respectively.

Techniques: Confocal Microscopy, Incubation, Labeling, Light Microscopy

Journal:

Article Title: Macrophage-Specific Lipid-Based Nanoparticles Improve MRI Detection and Characterization of Human Atherosclerosis

doi: 10.1016/j.jcmg.2008.08.009

Figure Lengend Snippet: Confocal microscopy merged images (NBD=green, anti-CD68=red, DAPI=blue) at 100X of atherosclerotic plaque incubated with (A) control (non-fluorescent untargeted NPs), (B) NBD-labeled untargeted NPs, (C) NBD-labeled Fc-NPs, (D) and NBD-labeled targeted NPs. The images were of the intimal plaque and therefore do not have the auto-fluorescence of the internal elastic lamina and media. There was no green fluorescence seen in control aortic plaque incubated with non-fluorescent untargeted NPs while non-specific distribution of NBD-labeled untargeted NPs, Fc-NPs, and targeted NPs can be seen throughout the plaque. An arrow is present in each image to identify resident macrophages. Significant colocalization was seen of NBD-labeled targeted NPs and anti-CD68 (D) while significant colocalization was not seen with NBD-labeled untargeted NPs and Fc-NPs (B & C). This suggests that macrophages are bound by targeted NPs targeting CD36.

Article Snippet: Biotinylated rabbit anti-human polyclonal antibodies to human CD36 (IgG, Novus Biologicals) and biotinylated ChromPure rabbit IgG, Fc fragment (Jackson Immunoresearch) were used to make targeted NPs s and Fc-NPs respectively.

Techniques: Confocal Microscopy, Incubation, Control, Labeling, Fluorescence